10% Neutral Buffered Formalin (NBF) -500ml Ready-to-Use Histological Tissue Fixative, Formaldehyde Wet Specimen Preservation, Research Chemical
BHD 11
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TriStains 10% NBF is well suited for large throughput laboratories, ensuring hassle-free tissue preservation and fixation without any additives required.
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Product Details
| % Assay of Chemical(s) | 10 |
| Item Form | Liquid |
| Purity Percentage | 10 |
| Item Weight | 2 Pounds (910 grams) |
| Volume | 500 Milliliters |
| Brand Name | TriStains |
| Product Grade | Reagent |
| Manufacturer | TriStains |
| UPC | 798339651498 |
| Model Number | TSL11758-500 |
| Part Number | TSL11758 |
| Included Components | NO |
| Brand | TriStains |
| Item Volume | 500 Milliliters |
| Percentage assay | 10 |
Dangerous Goods
To avoid the delay in customs clearance process for Dangerous Goods, the shipment shall be opened at our warehouse facility to check the severity of hazardous components. Please see the list of Dangerous goods here.
Who Should Buy?
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Histology Laboratories
Ideal for laboratories conducting histological studies requiring effective tissue fixation and preservation for microscopy.
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Medical Research
Highly suitable for researchers needing to fix biological specimens to study cellular structures and tissue pathology.
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Educational Institutions
Useful for biology and health sciences programs in schools and universities for teaching histology and anatomy.
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Home Users
Unsuitable for non-professionals or hobbyists due to safety concerns and the need for proper laboratory handling.
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Live Specimen Work
Not appropriate for applications involving live specimens, as it is designed for tissue fixation and preservation.
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Sensitive Environments
May not be suitable for settings requiring minimal chemical exposure or where volatile compounds are restricted.
Product Description
10% Neutral Buffered Formalin (NBF) -500ml Ready-to-Use Histological Tissue Fixative, Formaldehyde Wet Specimen Preservation, Research Chemical
Customer Questions & Answers
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Question:
What is 10% Neutral Buffered Formalin (NBF) used for?
Answer: 10% Neutral Buffered Formalin is primarily used as a histological tissue fixative in laboratories. It preserves biological tissues for microscopic examination, preventing decay and maintaining structural integrity. NBF neutralizes the effects of formaldehyde's acidity, offering a more stable solution for tissue preservation, which is vital when creating tissue slides for research purposes. It is widely employed in medical and educational institutions where precise tissue analysis is essential for accurate diagnoses and studies. -
Question:
How should 10% NBF be stored?
Answer: 10% NBF should be stored in a cool, well-ventilated area away from direct sunlight. Ideally, it should be kept in a tightly sealed container to prevent evaporation and contamination. Proper storage minimizes the risk of degradation of the solution, ensuring consistency and reliability in its use. Laboratories often store it in a dedicated chemical cabinet, keeping it organized with other histological reagents for easy access during procedures. -
Question:
Is 10% NBF safe to use?
Answer: While 10% NBF is a reliable fixative, it contains formaldehyde, which can be hazardous. It is important to use it in a well-ventilated space and wear appropriate personal protective equipment (PPE), such as gloves and goggles. Adequate precautions are required to mitigate health risks associated with exposure, making it essential for users to familiarize themselves with the Material Safety Data Sheet (MSDS) for NBF before working with it in the lab environment. -
Question:
What types of specimens can be preserved with 10% NBF?
Answer: 10% NBF is versatile and can be used to fix a variety of specimens, including biopsy samples, organ specimens, and other biological tissues. Its efficacy in preserving cellular morphology makes it ideal for educational specimens used in anatomy classes, or for diagnostic samples collected in clinical settings. Researchers using animal models can also utilize NBF to maintain tissue integrity during experiments, ensuring reliable results during microscopic evaluation. -
Question:
Can 10% NBF be used for long-term preservation?
Answer: While 10% NBF is excellent for initial preservation, it is generally recommended for short to intermediate-term preservation. For long-term storage, additional steps may be required, such as paraffin embedding, to maintain specimen integrity over extended periods. NBF can maintain tissue morphology for several days, but for projects requiring prolonged analysis or archiving, transitioning to a more stable preservation method is advisable. -
Question:
What is the pH level of 10% NBF and why is it important?
Answer: 10% NBF typically has a pH level of around 7.0, which is neutral. This pH is crucial because it helps prevent the precipitation of proteins and maintains the cellular structures during the fixation process. A neutral pH ensures that tissues are fixed without causing excessive shrinkage or distortion, which is essential for accurate diagnosis and histopathological analysis. This neutrality makes NBF a preferred choice in laboratories focusing on delicate tissue samples. -
Question:
How do I prepare tissue samples in 10% NBF?
Answer: To prepare tissue samples in 10% NBF, first, ensure that the tissue is adequately sized to fit within a container without compression. The ratio of fixative to tissue should typically be 10:1 to ensure sufficient penetration of the fixative. Place the tissue in the NBF solution immediately after collection to preserve cellular details effectively. This quick fixation step is vital for maintaining the morphology and preventing autolysis, ensuring the highest quality samples for microscopy. -
Question:
Can 10% NBF be used for animal tissues?
Answer: Yes, 10% NBF can be used effectively for animal tissues as well as human tissues. It plays a significant role in veterinary research and diagnostics, allowing for the preservation of organ samples from experimental animals. Biologists and veterinarians utilize NBF to prepare specimens for further analysis, ensuring that cellular structures remain intact for histological assessments. This versatility in application makes NBF an essential tool in both veterinary and medical laboratories. -
Question:
What is the shelf life of 10% NBF once opened?
Answer: Once opened, the shelf life of 10% NBF is generally around 6 months, depending on storage conditions and exposure to air. Users should ensure that the container is tightly sealed after each use to minimize contamination and evaporation. Conducting periodic checks for any changes in clarity or color can help assess the quality, allowing for the identification of potentially compromised solutions before use in critical applications. -
Question:
Where can I buy 10% Neutral Buffered Formalin NBF Ready-to-Use in Bahrain?
Answer: You can buy 10% Neutral Buffered Formalin NBF Ready-to-Use Histological Tissue Fixative from Ubuy. Ubuy offers a range of research chemicals, including NBF, with extensive details on the product specifications. Their platform allows you to explore different variants and acquire the necessary fixatives for your laboratory work or research needs.
TriStains Buffers TSL11758-500 Editorial Review
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Features & Benefits
- 10% Neutral Buffered Formalin (NBF) is ideal for light microscopy.
- Ready-to-use solution with no additives needed.
- Proven to maintain specimen integrity during lab analysis.
- Perfect for wet specimen preservation and large throughput laboratories.
- Reliable preservation for researchers and collectors on the go.
- Trusted for consistent performance in tissue fixation.