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Item #:
22349426
Whatman 10402506 Protran BA85 Nitrocellulose Blotting Membrane, Circle, 0.45m Pore Size, 25mm Diameter (Pack of 100)
Item #:
22349426
BHD 50
Price Details
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Imported from USA store
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Order now and get it around Saturday, July 18
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Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible.
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What Stands Out
High Quality Material
Crafted from premium nitrocellulose, this membrane ensures superior protein binding efficiency, enhancing the accuracy of your blotting experiments.
Optimal Pore Size
Featuring a 0.45µm pore size, it effectively retains proteins while allowing optimal transfer, making it ideal for various blotting applications.
Convenient Packaging
Packaged in sets of 100, these 25mm membranes provide excellent value, ensuring plenty of supplies for your research needs without sacrificing quality.
Product Details
| Item Weight | 1.5 lbs (680 grams) |
Who Should Buy?
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Molecular Biologists
Ideal for protein transfer and Western blotting procedures, ensuring high sensitivity and reliable results in experiments.
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Laboratory Technicians
Useful for routine laboratory applications, providing consistent results across various analytic techniques involving membrane blots.
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Research Institutions
Perfect for academic and commercial research environments requiring high-quality membranes for advanced protein analysis.
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Non-specialized Users
Individuals without lab training may struggle with proper handling and processing of nitrocellulose membranes effectively.
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Inexperienced Labs
New laboratories lacking sufficient protocols may face challenges using specific techniques suitable for this blotting membrane.
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Non-biolab Applications
Not suitable for applications outside of biological sciences, such as industrial or non-research-based uses of membranes.
Product Description
Whatman 10402506 Protran BA85 Nitrocellulose Blotting Membrane, Circle, 0.45m Pore Size, 25mm Diameter (Pack of 100)
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Customer Questions & Answers
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Question:
What is the purpose of the Whatman 10402506 Protran BA85 Nitrocellulose Blotting Membrane?
Answer: The Whatman 10402506 Protran BA85 Nitrocellulose Blotting Membrane is primarily used in molecular biology and biochemistry for the transfer of proteins and nucleic acids in various applications such as Western blotting and Southern blotting. Its porous structure allows for efficient binding and immobilization of biomolecules, ensuring high sensitivity and reliable results. Researchers commonly utilize this membrane for detecting antigen-antibody interactions or analyzing gene expressions, making it a crucial component in scientific studies. -
Question:
What are the dimensions and specifications of the Protran BA85 Nitrocellulose Blotting Membrane?
Answer: The Protran BA85 Nitrocellulose Blotting Membrane features a circular design with a diameter of 25mm and a pore size of 0.45 micrometers. These dimensions are optimized for effective blotting performance, allowing the transfer of smaller proteins while retaining sufficient binding capacity. The membrane's chemistry also ensures that proteins adhere firmly, enabling clear results without background noise, which is particularly important in sensitive analyses. -
Question:
How should I handle and store the Whatman 10402506 Nitrocellulose Blotting Membrane?
Answer: It is crucial to handle the Whatman 10402506 Nitrocellulose Blotting Membrane with care to avoid contamination. Store the membranes in a clean, dry environment at room temperature, preferably in a sealed container to protect them from moisture. Avoid direct sunlight and extreme temperatures, as these conditions may affect the membrane's performance. Proper storage ensures that the membranes retain their integrity for reliable use in various experimental setups. -
Question:
Can the Protran BA85 Nitrocellulose Blotting Membrane be used for various applications?
Answer: Yes, the Protran BA85 Nitrocellulose Blotting Membrane is versatile and can be used in multiple applications, including Western blotting, Southern blotting, and dot blot assays. Its robust features make it suitable for both protein and nucleic acid transfer processes. Laboratories often use this membrane for detection methods that include chemiluminescence and colorimetric assays, making it a popular choice in many research settings. -
Question:
What is the significance of pore size in the Protran BA85 Nitrocellulose Blotting Membrane?
Answer: The pore size of the membrane is critical as it influences the binding and retention of biomolecules. The 0.45 micrometer pore size is ideal for capturing a wide range of proteins and nucleic acids with varying sizes. This specific size ensures that while smaller molecules can pass through, larger proteins are effectively retained for detection. Thus, selecting the correct pore size is crucial for achieving the intended quality and sensitivity in experimental outcomes. -
Question:
Is the Whatman Protran BA85 Nitrocellulose Membrane suitable for high-throughput applications?
Answer: Absolutely, the Whatman Protran BA85 Nitrocellulose Membrane is designed to support high-throughput applications due to its consistent performance and reliability across multiple assays. The pack of 100 membranes is particularly useful for laboratories engaged in large-scale experiments or screenings, enabling researchers to process numerous samples simultaneously without compromising on results. This efficiency is essential for projects aimed at rapid discoveries or clinical diagnostics. -
Question:
How does the Protran BA85 Nitrocellulose Blotting Membrane compare to other membranes?
Answer: The Protran BA85 Nitrocellulose Blotting Membrane is often favored over other types of membranes such as PVDF due to its superior protein-binding capacity and lower background noise. Its nitrocellulose composition allows for easier transfer dynamics, resulting in clearer bands and spots during detection. Researchers typically choose this membrane when seeking high specificity and sensitivity in molecular biology applications, making it a trusted option in laboratories worldwide. -
Question:
What precautions should be taken while using the Whatman 10402506 Blotting Membrane?
Answer: When using the Whatman 10402506 Blotting Membrane, ensure that you work within a sterile environment to prevent contamination. Properly acclimatize the membrane to the transfer buffer before use to optimize binding efficiency. Additionally, handling the membrane with gloves can minimize direct contact and preserve its functionality. Following these precautions helps ensure high-quality results in your assays, maintaining the integrity of your samples throughout the process. -
Question:
Where can I buy the Whatman 10402506 Protran BA85 Nitrocellulose Blotting Membrane in Bahrain?
Answer: You can easily buy the Whatman 10402506 Protran BA85 Nitrocellulose Blotting Membrane from Ubuy in Bahrain. Ubuy offers a convenient platform for obtaining this essential laboratory product, ensuring that you can source it reliably for your research needs. With a user-friendly interface and various shipping options, Ubuy makes it straightforward to purchase the products necessary for your scientific endeavors. -
Question:
What should I consider when selecting a nitrocellulose membrane for my experiments?
Answer: When selecting a nitrocellulose membrane like the Protran BA85, consider factors such as pore size, thickness, and the specific application you are planning to undertake. The 0.45 micrometer pore size is suitable for a variety of applications, but for the best results, match the membrane's specifications with your proteins' characteristics. Additionally, evaluate your detection methods, as some membranes may perform better with specific types of chemiluminescent or dye-based assays.
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BHD 50
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Features & Benefits
- 100% pure nitrocellulose for optimal protein binding.
- Outstanding handling strength for various applications.
- Compatible with multiple detection methods.
- No methanol prewetting step required.
- Maintains molecular recognition activity for five years.
- Designed for high binding capacity and low background noise.