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Item #:
49688668
Tris-Glycine-SDS Buffer 10X, 500mL - The Curated Chemical Collection
Item #:
49688668
BHD 20
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What Stands Out
High Concentration
This 10X Tris-Glycine-SDS buffer offers a robust formulation, ensuring optimal performance for various electrophoresis applications, making it ideal for researchers seeking reliable results in their experiments.
Convenient Packaging
With a generous 500mL size, this buffer provides ample supply for multiple experiments, reducing the need for frequent reordering and ensuring that scientists can focus on their work without interruption.
Curated Quality
Part of a carefully selected chemical collection, this buffer meets high-quality standards, assuring users of its reliability and effectiveness, which is essential for achieving reproducible results in scientific research.
Product Details
| Item Weight | 1.1 lbs (500 grams) |
Dangerous Goods
To avoid the delay in customs clearance process for Dangerous Goods, the shipment shall be opened at our warehouse facility to check the severity of hazardous components. Please see the list of Dangerous goods here.
Who Should Buy?
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Biochemistry Students
Ideal for students conducting electrophoresis experiments in classes and labs, ensuring consistent and reliable buffer solutions.
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Research Laboratories
Essential for laboratories focusing on protein analysis, providing a stable environment for separation processes in various experiments.
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Molecular Biology Professionals
Suitable for professionals who require high-quality buffers for Western blotting and other molecular biology techniques.
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Home Users
Not suitable for casual or home users without access to appropriate lab equipment and safety measures.
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Non-Scientific Uses
The product is specifically designed for scientific applications, making it unsuitable for non-scientific or everyday tasks.
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Small Scale Experiments
Users needing minimal quantities may find the 500mL size excessive, leading to unnecessary waste for smaller projects.
Product Description
Tris-Glycine-SDS Buffer 10X, 500mL - The Curated Chemical Collection
About This Item
Introducing Tris-Glycine-SDS Buffer 10X, 500mL from The Curated Chemical Collection. This premium chemical product is specifically designed to meet the needs of educational labs dealing with genetics and molecular biology. Our 10X tris-glycine-SDS buffer is a crucial component for protein separation, ensuring accurate and reliable results every time.
Manufactured in the United States, you can trust the quality and performance of this chemical. Each 500mL bottle comes with clear and concise handling and storage procedures printed directly on the label, ensuring safe and proper usage. We prioritize the safety of our customers, which is why our product is labeled "For Laboratory Use Only." Please note that this product is not intended for drug, food, or household use, and should be kept out of reach of children. As part of our commitment to transparency, a Safety Data Sheet is available for your reference. We understand the importance of providing comprehensive information to our customers, enabling you to make informed decisions. Choose The Curated Chemical Collection for all your chemical needs.
Whether you're an educator, researcher, or avid scientist, our curated selection of chemicals is tailored to meet your requirements. With our chemical collection, we strive to provide a reliable and convenient solution for your e-commerce needs. Trustworthy and responsive, The Curated Chemical Collection is your go-to chemical supplier for e-commerce platforms. Browse our extensive inventory of curated chemicals, hand-picked to meet the demands of laboratories and scientific exploration.
Invest in the best with The Curated Chemical Collection. We prioritize quality, customer satisfaction, and safety, making us a trusted chemical supplier in the e-commerce marketplace. Explore our online store today and discover a wide range of chemical solutions for your research and lab needs. Choose The Curated Chemical Collection, your trusted partner in chemical trading for e-commerce.
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Customer Questions & Answers
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Question:
What is the primary use of ALDON Innovating Science Tris-Glycine-SDS Buffer 10X?
Answer: The primary use of ALDON Innovating Science Tris-Glycine-SDS Buffer 10X is for protein electrophoresis. This buffer helps maintain a stable pH during the separation of proteins according to their size. It is particularly effective in polyacrylamide gel electrophoresis (PAGE) protocols. By utilizing this buffer, researchers can achieve high-resolution separation of proteins, which is crucial for subsequent analysis, such as Western blotting or mass spectrometry. -
Question:
How should I dilute ALDON Tris-Glycine-SDS Buffer for use?
Answer: To prepare the working solution, dilute the 10X buffer with distilled water at a 1:10 ratio, meaning one part buffer to nine parts water. This results in a 1X solution that is suitable for use in electrophoresis experiments. Proper dilution is vital to ensure consistent results across experiments and to maintain the integrity of your samples during analysis. -
Question:
What benefits does using a tris-glycine buffer provide?
Answer: Using a tris-glycine buffer provides several benefits, including a stable pH environment essential for protein separation. This buffer system effectively enhances the resolution and sharpness of bands in gel electrophoresis. It also allows for versatile application across various protein types, leading to more reliable and reproducible results in biochemical assays and research studies. -
Question:
Is the ALDON Tris-Glycine-SDS Buffer suitable for all types of proteins?
Answer: Yes, the ALDON Tris-Glycine-SDS Buffer is suitable for a wide range of proteins, including both hydrophilic and hydrophobic samples. The SDS component helps denature proteins by disrupting their secondary and tertiary structures, allowing for size-based separation during electrophoresis. It is commonly used for analyzing protein expression levels and molecular weight determination, making it a versatile choice for researchers. -
Question:
What storage conditions are recommended for this buffer?
Answer: It is recommended to store the ALDON Innovating Science Tris-Glycine-SDS Buffer at room temperature, away from direct sunlight and extreme temperature fluctuations. Proper storage helps maintain the buffer's effectiveness and ensures optimal performance during experiments. Always tightly close the container to prevent contamination and evaporation, which could affect the buffer’s composition. -
Question:
Can I use this buffer for DNA electrophoresis?
Answer: No, ALDON Tris-Glycine-SDS Buffer is specifically formulated for protein electrophoresis. For DNA electrophoresis, other buffers like TAE or TBE are more appropriate. Each buffer system has unique properties tailored to optimize the behavior of nucleic acids or proteins during electrophoresis, hence it's important to choose the right one for your specific applications. -
Question:
What is the significance of using SDS in this buffer?
Answer: The inclusion of SDS (sodium dodecyl sulfate) in the buffer is critical as it provides a negative charge to proteins, ensuring uniform migration during electrophoresis. SDS denatures proteins, allowing their size to dictate movement through the gel matrix. This characteristic is essential for accurate molecular weight determination and for analyzing protein purity and composition in various research applications. -
Question:
How can I ensure consistent results when using this buffer?
Answer: To ensure consistent results, always use fresh dilutions of the buffer and verify that your electrophoresis setup is calibrated correctly. Additionally, running a protein ladder alongside samples can help you accurately assess molecular weight and consistency across different experiments. Following standardized protocols may also help maintain reproducibility. -
Question:
What types of experiments can benefit from using this buffer?
Answer: Experiments that involve protein purification, characterization, and analysis can greatly benefit from using the ALDON Tris-Glycine-SDS Buffer. Common applications include Western blotting, gel filtration, and SDS-PAGE. It's suitable for academic research, industrial applications, and quality control testing, aiding in the development and verification of therapeutic proteins and other biomolecules. -
Question:
Where can I buy ALDON Innovating Science Tris-Glycine-SDS Buffer 10X, 500mL?
Answer: You can buy ALDON Innovating Science Tris-Glycine-SDS Buffer 10X, 500mL from Ubuy. Ubuy is a reliable e-commerce platform that offers a wide range of chemicals and laboratory supplies, making it easy for you to find the products you need for your scientific research and experiments.
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Pros
- Highly effective for gel electrophoresis
- Great buffer stability
- Convenient 10X concentration
- consistently delivers reliable results
- User-friendly for lab applications
Cons
- The bottle design could be improved.
Product Price History
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BHD 20
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