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منتج #:
145280186
Tris Buffer, pH 8.5 [1M] - 125 mL Volume
82% من المشترين سيوصون بهذا المنتج لصديق
منتج #:
145280186
BHD 20
تفاصيل السعر
باستثناء رسوم الشحن والجمارك ( سيتم احتساب رسوم الشحن والجمارك عند إتمام الشراء )
*سيتم استيراد جميع العناصر من أمريكا
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مستورد من متجر USA
كمية:
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أفضل شركائنا اللوجستيين
1M Tris Buffer pH 8.0 is a ready-to-use solution essential for your molecular biology and proteomics applications.
شحن
سريع
استرجاع
مجاني*
تغليف آمن
منتجات أصلية %100
الامتثال لمعيار PCI DSS
حاصل على شهادة ISO 27001
Note: Step Down Voltage Transformer required for using electronics products of أمريكا store (110-120). Recommended power converters اشتري الآن.
مايفيد
Standardization
This Tris Buffer offers a consistent pH of 8.5, ensuring reliable results in biochemical experiments and reactions that require precise buffering capacity.
High Concentration
With a 1M concentration, this buffer provides ample capacity to maintain stable pH levels in various applications, making it suitable for high-demand laboratory needs.
Convenient Volume
Packaged in a 125 mL bottle, it strikes a balance between sufficient quantity for experiments and usability, preventing waste while catering to both small and medium-scale applications.
تفاصيل المنتج
| Container Type | Bottle |
| Item Form | Liquid |
| Item Weight | 0.25 Pounds (110 grams) |
| Volume | 125 Milliliters |
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Part Number | 23.105.35 |
| Included Components | Bottle |
| Brand | eCon Lab Supply |
| Item Volume | 125 Milliliters |
| Package Information | Bottle |
من يجب أن يشتري؟
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Biochemistry Labs
Ideal for researchers conducting experiments that require stable pH conditions in biochemical reactions.
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PCR Applications
Useful for molecular biologists performing PCR, as it helps maintain optimal pH during DNA amplification.
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Cell Culture
Perfect for cell biologists needing a buffer for maintaining pH in cell culture mediums.
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Acidic Solutions
Not suitable for applications requiring acidic conditions, as Tris buffer is not effective below pH 7.
وصف المنتج
Tris Buffer, pH 8.5 [1M] - 125 mL Volume
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أسئلة العملاء & الإجابات
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سؤال:
What is Tris buffer and what is its purpose?
إجابه: Tris buffer, also known as Tris(hydroxymethyl)aminomethane, is a widely used buffering agent in biological and chemical applications. Its primary purpose is to maintain a consistent pH during experiments, particularly in molecular biology, biochemistry, and electrophoresis. Tris buffer at pH 8.5 is particularly ideal for experiments requiring basic conditions, ensuring that enzymes and proteins remain stable. For instance, researchers often employ this buffer when conducting PCR reactions or protein assays, where precise pH control can significantly affect the outcomes. -
سؤال:
How do I prepare Tris buffer at pH 8.5?
إجابه: Preparing Tris buffer at pH 8.5 is straightforward. Start with Tris base and deionized water, then adjust the pH by adding hydrochloric acid (HCl) until you reach the desired level. For a ready-to-use solution, you can purchase pre-made Tris buffer like the 1M version in a 125 mL volume. This convenience eliminates the need for complex calculations and ensures consistency across experiments, making it ideal for time-sensitive work like gel electrophoresis, where reliable pH is essential for separation processes. -
سؤال:
Is Tris buffer safe to use in the lab?
إجابه: Yes, Tris buffer is generally regarded as safe for laboratory use when handled with appropriate lab safety protocols. It has low toxicity, making it favorable for many biological applications. However, as it is a chemical reagent, it's important to avoid direct contact with skin and eyes and to work in a well-ventilated area. In practical scenarios, researchers often utilize Tris buffer for sensitive experiments involving DNA, RNA, or proteins, ensuring a safe environment while yielding reliable results in their studies. -
سؤال:
What applications is Tris buffer used for?
إجابه: Tris buffer is highly versatile and is used in a variety of applications spanning molecular biology, protein synthesis, and electrophoresis. It's commonly applied in DNA and RNA extraction processes, as well as in the preparation of agarose gels for electrophoresis. This buffer is also used in cell culture and enzymatic reactions where maintaining a stable pH is crucial. For example, in Western blotting, Tris buffer helps to ensure the integrity of protein separation, enhancing assay reproducibility and accuracy. -
سؤال:
Can Tris buffer be used with all types of samples?
إجابه: While Tris buffer is suitable for a wide range of biological samples, it's important to note that it may not be compatible with all. Certain proteins or enzymatic reactions can be sensitive to Tris, which may interfere with assay results. For example, if conducting a project that involves proteins sensitive to basic pH, it’s advisable to evaluate alternative buffers. Researchers often select Tris buffer for nucleic acid work or protein studies, given its broad applicability and reliable performance in these scenarios. -
سؤال:
How long can I store Tris buffer?
إجابه: Tris buffer can typically be stored for a prolonged period, often up to several months if kept under proper conditions. It should be stored in a cool, dark place and ideally in a tightly sealed container to prevent contamination and evaporation. However, it is crucial to regularly check for any color changes or precipitation, which can indicate degradation. In laboratory contexts, it is common for scientists to prepare fresh buffer solutions as needed for critical experiments to ensure optimal performance. -
سؤال:
What is the significance of the pH level in Tris buffer?
إجابه: The pH level in Tris buffer is crucial as it impacts the stability and activity of biological molecules, such as proteins and nucleic acids. A pH of 8.5 is optimal for many biochemical applications because it closely resembles physiological conditions in many organisms. Changes in pH can lead to denaturation or loss of activity in sensitive applications. Therefore, when using Tris buffer in experiments such as enzyme assays or DNA amplification, maintaining the precise pH is essential for achieving reliable results. -
سؤال:
Can Tris buffer be used for preparing gels?
إجابه: Absolutely, Tris buffer is commonly used in preparing agarose and polyacrylamide gels for electrophoresis. Its ability to maintain a stable pH during the electrophoretic process is essential for consistent banding patterns and resolution. When preparing these gels, scientists typically mix Tris buffer with agarose or acrylamide, depending on the type of samples they're working with. This practice is fundamental in molecular biology labs for analyzing DNA, RNA, or proteins, facilitating clear visualization and accurate separation during analysis. -
سؤال:
Where can I buy Tris Buffer, pH 8.5 [1M] - 125 mL Volume?
إجابه: You can purchase Tris Buffer, pH 8.5 [1M] - 125 mL Volume from Ubuy in Bahrain. Ubuy provides a convenient online shopping experience, ensuring that you have access to quality laboratory products like Tris buffer. They often stock various sizes and concentrations, allowing you to choose the most suitable option for your research needs. With Ubuy's extensive range and reliable service, it's an excellent choice for obtaining your lab supplies efficiently.
eCon Lab Supply Buffers Editorial Review
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BHD 20
اطلب الآن واحصل عليه حول الثلاثاء, سبتمبر 22
هذا المنتج غير ممنوع في بلدي. (الرجاء الضغط على الرابط أعلاه إذا لم يكن هذا المنتج ممنوعاً في بلدك ، لذلك سيقوم فريقنا بمراجعته والسماح به.)
كمية:
معاملة آمنة
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المميزات والفوائد
- Comprehensive range of biological buffers for research and diagnostics.
- Manufactured in an ISO certified facility in the USA.
- Strict adherence to Good Manufacturing Practices and quality control.
- 1M Tris Buffer pH 8.0 is ready-to-use and versatile.
- Prepared with high-quality molecular biology grade ingredients.
- Suitable for both DNA and RNA applications.
ضمان Ubuy
تسوّق بثقة مع منتجات أصلية %100، ومدفوعات آمنة متوافقة مع معيار PCI DSS، وحماية بيانات معتمدة وفق ISO 27001، وشحن دولي سريع، وإرجاع مجاني*، وتغليف آمن لكل طلب.