Citrate Buffer 0.2 m, pH 4.0, 1 L
Citrate buffers can be used for RNA isolation and are a popular choice for immunofluorescent staining applications.
Citrate Buffer 0.2 m, pH 4.0, 1 L
منتج #: 145286292

Citrate Buffer 0.2 m, pH 4.0, 1 L

منتج #: 145286292

BHD 22

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Citrate buffers can be used for RNA isolation and are a popular choice for immunofluorescent staining applications.
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مايفيد

Consistent pH Levels
This citrate buffer maintains a stable pH of 4.0, ensuring reliability in biochemical experiments, essential for accurate results in various applications.
High Concentration
With a concentration of 0.2 M, this buffer provides effective buffering capacity, making it ideal for lab settings requiring precise conditions for enzyme reactions and other sensitive processes.
Large Volume
At 1 liter, this citrate buffer is perfect for extensive laboratory projects, reducing the need for frequent replenishment and saving time and resources in the lab environment.

تفاصيل المنتج

Shop Citrate Buffer 0.2 m, pH 4.0, 1 L online at a best price in البحرين. B09V5N131L
Item FormLiquid
Item Weight1.25 Pounds (570 grams)
Volume1 Liters
Brand NameeCon Lab Supply
ManufacturerCiro Mfg
Part Number23.105.131
Included ComponentsBottle
BrandeCon Lab Supply
Item Volume1 Liters

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Suitable For
  • Biological Research

    Ideal for experiments requiring a stable pH, such as enzymatic reactions and protein studies in biological research.

  • Laboratory Use

    Useful in various laboratory applications like chromatography, providing reliable results with consistent pH control.

  • Sample Preservation

    Effectively stabilizes samples, preventing degradation and maintaining integrity during experiments or long-term storage.

Not Suitable For
  • High pH Requirements

    Unsuitable for applications needing a higher pH, as its formulated pH is specifically low at 4.0.

وصف المنتج

Citrate Buffer 0.2 m, pH 4.0, 1 L

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أسئلة العملاء & الإجابات

  • سؤال: What is Citrate Buffer 0.2 m, pH 4.0 used for?

    إجابه: Citrate Buffer 0.2 m, pH 4.0 is widely used in biological and biochemical applications, including enzyme reactions, protein purification, and maintaining the pH of samples in laboratory experiments. Its ability to stabilize pH levels makes it ideal for experiments requiring precise measurements, particularly in molecular biology techniques. For instance, in the process of electrophoresis, maintaining a stable pH is crucial to achieving accurate results.
  • سؤال: How is Citrate Buffer prepared?

    إجابه: Citrate Buffer can be prepared by mixing citric acid and its sodium salts in a specific ratio to achieve the desired pH. For a 0.2 m concentration, carefully use a precise amount of citric acid and sodium citrate, followed by adjusting the pH with dilute sodium hydroxide or hydrochloric acid. Regular monitoring with a pH meter is essential to ensure accuracy. This preparation method is particularly useful in laboratory settings where customized buffers are essential for specific assays or reactions.
  • سؤال: Is Citrate Buffer suitable for cell culture applications?

    إجابه: Yes, Citrate Buffer is suitable for various cell culture applications, especially when maintaining cell pH is crucial. Its phosphate-free formulation minimizes toxicity and supports the stable growth of sensitive cell lines. Researchers often use it during the preparation of culture media or during the maintenance phase to ensure optimal growth conditions, making it an essential component in cellular assays and biotechnology studies.
  • سؤال: What is the importance of pH 4.0 in Citrate Buffer?

    إجابه: A pH of 4.0 in Citrate Buffer is significant because it falls within the optimal range for various biological reactions, particularly those involving enzymes that are active in acidic environments. This specific pH allows researchers to study enzyme kinetics, protein interactions, and other biochemical processes effectively. For example, many enzymes, such as pepsin, work best in acidic conditions, making this buffer ideal for experiments that require such environments.
  • سؤال: Can Citrate Buffer be used in protein analysis?

    إجابه: Absolutely, Citrate Buffer is often employed in protein analysis, as it helps maintain a stable pH during the experimental procedure. Its compatibility with various proteins allows for effective analysis in techniques like chromatography and gel electrophoresis. Researchers frequently use this buffer to isolate and study proteins, as maintaining a consistent pH is crucial for preserving protein structure and function throughout the analysis.
  • سؤال: How should Citrate Buffer be stored?

    إجابه: Citrate Buffer should be stored in a cool, dry place, ideally at room temperature and protected from direct sunlight. It is important to keep it in a tightly sealed container to prevent contamination and evaporation. Proper storage ensures its stability and effectiveness for later use, especially in critical laboratory experiments. Regular checks on the buffer’s clarity and pH are also advisable before each use to ensure optimal performance during experiments.
  • سؤال: What are the safety precautions when handling Citrate Buffer?

    إجابه: When handling Citrate Buffer, it is essential to use personal protective equipment such as gloves and goggles to prevent skin or eye irritation. Although it's relatively safe, it's important to avoid ingestion and to work in a well-ventilated area. Always follow laboratory safety protocols, and check material safety data sheets (MSDS) for specific handling instructions. These precautions ensure safe use during various biochemical applications and experiments.
  • سؤال: Is Citrate Buffer compatible with other lab reagents?

    إجابه: Citrate Buffer is generally compatible with a wide range of laboratory reagents, making it versatile for numerous applications. However, it's important to consider the specific chemistry of other reagents used in conjunction, as some compounds may alter the pH or stability of the buffer. Researchers often test compatibility beforehand, especially in multi-reagent experiments, ensuring that the buffer maintains its effectiveness while achieving desired outcomes.
  • سؤال: How does the concentration of Citrate Buffer affect its function?

    إجابه: The concentration of Citrate Buffer directly influences its buffering capacity and effectiveness in maintaining pH during biochemical reactions. A 0.2 m concentration provides robust buffering ability, suitable for most biological applications. Adjusting the concentration allows researchers to tailor the buffer to specific experiments and conditions, ensuring that enzymatic reactions proceed optimally without significant pH fluctuations, which is critical for precise scientific work.
  • سؤال: Where can I buy Citrate Buffer 0.2 m, pH 4.0, 1 L in Bahrain?

    إجابه: You can purchase Citrate Buffer 0.2 m, pH 4.0, 1 L from Ubuy, which offers a reliable platform for sourcing this product in Bahrain. Ubuy provides a user-friendly shopping experience with a variety of laboratory supplies at competitive prices.

eConLabSupply Buffers Editorial Review

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